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KMID : 1145120200180030271
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Asian Journal of Beauty and Cosmetology
2020 Volume.18 No. 3 p.271 ~ p.291
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Anti-oxidant and Moisturizing Effects of Oil Extracted from Tenebrio molitor Larvae
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Kim Hae-Ok
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Abstract
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Purpose : Recent widespread research into the biological effects of edible insects has occurred because they place a low burden on the environment, and the output offsets the effort. However, the anti-oxidant and moisturizing effects of their oils are relatively unknown. Tenebrio molitor larvae possess anti-adipogenic effects. This study investigated the anti-oxidant and moisturizing effects of Tenebrio molitor larvae oil (TMO) on HaCaT keratinocytes.
Methods : We used a supercritical fluid extraction method to extract oil from Tenebrio molitor larvae. After purification, we evaluated the anti-oxidant activity of the TMO according to its 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reduction potential, and metal-chelating activity. An 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the cell viability of HaCaT keratinocytes treated with TMO. Reverse transcription-polymerase chain reaction assays were used to analyze the expression levels of ceramide synthase and ceramidase in TMO-treated HaCaT keratinocytes.
Results : Treatment with 0.01%?0.2% TMO effectively scavenged DPPH radicals. In line with this, the TMO treatment showed a dose-dependent increase in reduction potential and metal-chelating activities. TMO treatment at concentrations of 0.01%-0.2% up to 0.2% for 72 h did not affect the viability of HaCaT keratinocytes. TMO treatment of HaCaT keratinocytes at nontoxic concentrations significantly upregulated the expression of ceramide synthase mRNA and downregulated the expression of ceramidase mRNA.
Conclusion : TMO possesses anti-oxidant and moisturizing effects that show potential for use health products and cosmetics.
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KEYWORD
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Tenebrio molitor larvae, Anti-oxidant, Oxidative stress, Skin, Ceramide
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